Herein, we reported for the first time one step procedure for the preparation of cytochrome c (cyt c)-poly
(5-amino-2-napthalenesulfonic acid) (PANS) modified glassy carbon electrode by cyclic voltammetrically
(CV). Hereafter, we called the above modified electrode as cyt c-PANS electrode. The presence of cyt c on
modified electrode was investigated with electrochemical quartz crystal microbalance (EQCM), CV, and
superoxide radicals reaction studies. The reaction between cyt c in the modified electrode and superoxide
radicals in solution, was exemplified by cyclic voltammetric measurements. Surface morphology of the
modified electrode was investigated by using atomic force microscopy (AFM). The modified electrode
showed a pair of well defined redox peak in PBS solution, pH 6.7. The modified electrode utilized for electrocatalytic
reduction aswell as amperometric determination of hydrogen peroxide (H2O2). The detection
limit and linear range for H2O2 were 5 and 50M to 7mM, respectively.